Nyquist Calculator

Find the optimal Nyquist pixel size for your microscope, by comparing the camera resolution to the microscope resolution.

Nyquist Pixel Size Calculator

Wavelength (nm):

Enter the wavelength for imaging

Objective Magnification:

Enter the magnification of your objective in x (e.g., 60x)

Numerical Aperture (NA):

Enter the Numerical Aperture of the lens

Additional Lens:

Enter the magnification of any additional lens

Pixel Size (μm):

Enter the pixel size of the camera in a given mode