"The Dhyana 400BSI V3 runs at the speed and sensitivity we need for lightsheet microscopy, and we have built our own control software using the Tucsen SDK."

- Ludovico Silvestri, Silvestri Group,
European Laboratory for Non-linear Spectroscopy (LENS), University of Florence

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Group Research Aims

The Silvestri group develops advanced light-sheet fluorescence microscopy (LSFM) methods to uncover how brain circuits encode behaviour. Their research integrates tissue clearing, high-resolution optical imaging, and computational analysis to achieve whole-brain mapping at cellular resolution. By creating scalable tools such as BRANT, they quantify neuronal activation patterns during learning, memory, and emotional processing. Functional analysis is paired to anatomical mapping of single axons by imaging expanded samples. The group’s goal is to link microscopic neural activity with macroscopic brain function, revealing how distributed networks change across states and between sexes. Through continuous innovation in imaging and data analysis, the lab strives to push the boundaries of systems neuroscience and brain-wide mapping.

Group Website

Equipment & Experiment

In addition to neuroscience, the Silvestri group is currently expanding the application of light-sheet microscopy to understand the complex morpho-chemical organization of cancer tissue in human biopsies.

To this aim, they are devising new methods to reduce spectral cross-talk in light-sheet fluorescence imaging, achieving a multiplexed view of the sample at the same throughput of the single-channel LSFM system. They develop and integrate custom hardware and software to achieve these ambitious technological goals.

Images are adapted from those provided by the Silvestri lab.

Experience with Tucsen

"The development of our research was previously limited by the time needed to collect data from large tissue sections. Working with Tucsen was straightforward: we demoed several cameras, selected the best for field of view and speed, received a same-day quote, and the camera was delivered on time and even installed on-site. The highly sensitive and fast readout of the Dhyana 400BSI V3 enables quicker data collection without sacrificing signal level, and line time delay mode enables straightforward integration into our system."

- Ludovico Silvestri, LENS, University of Florence

Dhyana 400BSI V3

The Dhyana 400BSI V3 features the GSENSE2020BSI sensor delivering sCMOS sensitivity with large fields of view

  • 95% Peak QE
  • 100 fps
  • 1.1 e- Read Noise
  • 4 Million Pixels
  • 6.5 Micron Pixels
  • USB 3.0 and CameraLink

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