“Our methods for high-throughput imaging of molecular processes requires both high sensitivity and speed. The Dhyana 2100's combination of high frame rate, high sensitivity and large detector enables the capture of biomolecular reactions with unprecedented precision, without compromising statistics.”

- Thomas Bugea, Dulin Lab, VU Amsterdam



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Group Research Aims

The Dulin lab at Vrije Universiteit (VU) Amsterdam develops and applies advanced single-molecule biophysics approaches such as magnetic tweezers, total internal reflection fluorescence (TIRF) microscopy and their combination, to investigate proteins-nucleic acids interactions such as polymerases and helicases.

Group Website

Equipment & Experiment

The group uses a custom high-throughput magnetic tweezers apparatus. This enables direct tracking of enzyme activity and conformational changes of nucleic acids & their associated proteins at nanometer, millisecond resolution for molecules tethered between the magnetic bead and the glass surface.

This setup allows the analysis of hundreds of beads with subnanometer resolution using the camera's FastBinning mode: 9.0 μm pixels at ~1 kHz frame rate.

These methods can be used to study many biological processes, such as polymerase elongation dynamics, nucleic acid unwinding by helicases, and nucleic acid mechanical properties under force and torque, with unprecedented resolution.

Experience with Tucsen

“We really appreciate the continued support that we have had for implementing the camera into our custom software using the Tucsen SDK. It is an excellent instrument for sensitivity at kilohertz frame rates, and we are actively looking to upgrade our other setups to have this ability for high throughput & high resolution as well.” – David Dulin, VU Amsterdam

Dhyana 2100

The Dhyana 2100 features the GSPRINT4521 sensor, allowing for high speed, large FOV imaging over 21 megapixels.

  • 450 fps in 8-bit, 975 fps in 12-bit Bin2
  • 3.5 e- Read Noise
  • 21 Million Pixels
  • 4.5 Micron Pixels
  • CXP12 x8