“We were previously limited using CCD cameras which required a few seconds to acquire & transfer data in our high-throughput setup. With the Dhyana 95 V2, this process is done in milliseconds with comparable quantum efficiency.”

- Jian Wang, Canadian Light Source



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Group Research Aims

The Canadian Light Source is Canada’s national synchrotron radiation facility, located at the University of Saskatchewan. The Spectromicroscopy (SM) beamline produces bright, tunable soft X-rays to support fundamental & applied research in health, materials, and environment for research groups in Canada & around the world using scanning transmission X-ray microscopy (STXM), X-PEEM, and others.

Dr Jian Wang is a Senior Scientist, Beamline Responsible for the SM beamline.

Group Website
Coherent diffractive imaging & ptychography method diagram at Canadian Light Source

Equipment & Experiment

The group uses scanning transmission X-ray microscopy (STXM) in both ambient & cryogenic conditions.

In ambient STXM using the Dhyana 95 V2's high dynamic range (HDR) mode, they can capture soft X-ray directly-produced electron wells up to 80 ke- in 16-bit, allowing for great dynamic range, and liquid cooling to ensure minimal background.

The sensor detects diffracted soft X-rays in an overlapped raster scan pattern of zone plate projection in a process known as ptychography, the scanning mode of coherent diffractive imaging (CDI). The diffractive image data is transferred and inverse Fourier transformed to reconstruct real-space images with 5.6 nm resolution: a more than 7-fold improvement over the conventional STXM-based nanoprobe methods (40 nm).

These instruments enable the use of 2D & 3D spectro-ptychography for spatially-resolved studies of nanoscale morphology in materials science, environmental science, and related fields.

The group also uses their own custom Python-based software for acquisition and ptychography data reconstruction in a high-throughput pipeline.

Experience with Tucsen

“The price & quality of the camera is impressive. I would expect this kind of quality sCMOS at around $50k, yet we paid far less.

Setting up the camera was very straightforward and we got great technical support from Tucsen for both hardware and custom software implementation. We are looking to upgrade our other systems to use the Dhyana, also.” – Jian Wang, CLS

Dhyana 95 V2 Tucsen camera

Dhyana 95 V2

The Dhyana 95 V2 features the GSENSE400BSI sensor, delivering sCMOS sensitivity with large fields of view.

  • 95 % Peak QE
  • 48 fps
  • 1.6 e- Read Noise
  • 4.2 Million Pixels
  • 11 Micron Pixels
  • CameraLink & USB 3.0